flow meter model t402 Search Results


flow meter model t402  (Transonic Systems Inc)
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Transonic Systems Inc flow meter model t402
Flow Meter Model T402, supplied by Transonic Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transonic ® flow meter  (Transonic Systems Inc)
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Transonic Systems Inc transonic ® flow meter
Transonic ® Flow Meter, supplied by Transonic Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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electromagnetic flowmeter t402 2-channel modular flow meter  (Transonic Systems Inc)
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Transonic Systems Inc electromagnetic flowmeter t402 2-channel modular flow meter
Electromagnetic Flowmeter T402 2 Channel Modular Flow Meter, supplied by Transonic Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transonic flow probe 12psb521  (Transonic Systems Inc)
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Transonic Systems Inc transonic flow probe 12psb521
Transonic Flow Probe 12psb521, supplied by Transonic Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ultrasound transit time flow meters ts410  (Transonic Systems Inc)
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Transonic Systems Inc ultrasound transit time flow meters ts410
Ultrasound Transit Time Flow Meters Ts410, supplied by Transonic Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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model t402 transonic systems  (ADInstruments)
86
ADInstruments model t402 transonic systems
Model T402 Transonic Systems, supplied by ADInstruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paclitaxel (t402)  (Millipore)
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Millipore paclitaxel (t402)
(A) Representative images of untransfected SKOV-3 cells and SKOV-3 cells transfected with siRNA targeting MAD2 (MAD2 siRNA) or a scrambled negative control siRNA (Scr siRNA) for 72 hours. After 24 hours, cells were either left untreated (-PTX) (a-c) or were treated with a 1μM dose of <t>paclitaxel</t> (+PTX) (d-f) for a further 48 hours. (B) Representative images of SKOV-3 cells stained using the senescence β-galactosidase staining kit following transfection for 72 hours (a-c) or 120 hours (d-f). (C) CCK-8 assay results. % cell viability for each condition was calculated as a percentage of untransfected SKOV-3 cells which were left untreated. The results demonstrate that knockdown of MAD2 renders SKOV-3 cells resistant to paclitaxel. (D) The percentage of β-galactosidase positive cells was calculated for each condition for (n = 3) technical and (n = 3) biological replicates. Following transfection a 3-fold increase in β-galactosidase expression was observed demonstrating that knockdown of MAD2 induces cellular senescence in SKOV-3 cells. (E) Trypan blue exclusion assay. (F) CCK-8 assay results for SKOV-3 cells treated with 200μM carboplatin for 48 hours following a 24 hour transfection. Results are expressed as mean +/-SD, n = 3. *p<0.05, **p<0.01, ***p<0.001 (Student’s t-test).
Paclitaxel (T402), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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an eight-channel powerlab  (ADInstruments)
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ADInstruments an eight-channel powerlab
(A) Representative images of untransfected SKOV-3 cells and SKOV-3 cells transfected with siRNA targeting MAD2 (MAD2 siRNA) or a scrambled negative control siRNA (Scr siRNA) for 72 hours. After 24 hours, cells were either left untreated (-PTX) (a-c) or were treated with a 1μM dose of <t>paclitaxel</t> (+PTX) (d-f) for a further 48 hours. (B) Representative images of SKOV-3 cells stained using the senescence β-galactosidase staining kit following transfection for 72 hours (a-c) or 120 hours (d-f). (C) CCK-8 assay results. % cell viability for each condition was calculated as a percentage of untransfected SKOV-3 cells which were left untreated. The results demonstrate that knockdown of MAD2 renders SKOV-3 cells resistant to paclitaxel. (D) The percentage of β-galactosidase positive cells was calculated for each condition for (n = 3) technical and (n = 3) biological replicates. Following transfection a 3-fold increase in β-galactosidase expression was observed demonstrating that knockdown of MAD2 induces cellular senescence in SKOV-3 cells. (E) Trypan blue exclusion assay. (F) CCK-8 assay results for SKOV-3 cells treated with 200μM carboplatin for 48 hours following a 24 hour transfection. Results are expressed as mean +/-SD, n = 3. *p<0.05, **p<0.01, ***p<0.001 (Student’s t-test).
An Eight Channel Powerlab, supplied by ADInstruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho-pak1 t423 antibody  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc phospho-pak1 t423 antibody
(A) Representative images of untransfected SKOV-3 cells and SKOV-3 cells transfected with siRNA targeting MAD2 (MAD2 siRNA) or a scrambled negative control siRNA (Scr siRNA) for 72 hours. After 24 hours, cells were either left untreated (-PTX) (a-c) or were treated with a 1μM dose of <t>paclitaxel</t> (+PTX) (d-f) for a further 48 hours. (B) Representative images of SKOV-3 cells stained using the senescence β-galactosidase staining kit following transfection for 72 hours (a-c) or 120 hours (d-f). (C) CCK-8 assay results. % cell viability for each condition was calculated as a percentage of untransfected SKOV-3 cells which were left untreated. The results demonstrate that knockdown of MAD2 renders SKOV-3 cells resistant to paclitaxel. (D) The percentage of β-galactosidase positive cells was calculated for each condition for (n = 3) technical and (n = 3) biological replicates. Following transfection a 3-fold increase in β-galactosidase expression was observed demonstrating that knockdown of MAD2 induces cellular senescence in SKOV-3 cells. (E) Trypan blue exclusion assay. (F) CCK-8 assay results for SKOV-3 cells treated with 200μM carboplatin for 48 hours following a 24 hour transfection. Results are expressed as mean +/-SD, n = 3. *p<0.05, **p<0.01, ***p<0.001 (Student’s t-test).
Phospho Pak1 T423 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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powerlab  (ADInstruments)
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ADInstruments powerlab
(A) Representative images of untransfected SKOV-3 cells and SKOV-3 cells transfected with siRNA targeting MAD2 (MAD2 siRNA) or a scrambled negative control siRNA (Scr siRNA) for 72 hours. After 24 hours, cells were either left untreated (-PTX) (a-c) or were treated with a 1μM dose of <t>paclitaxel</t> (+PTX) (d-f) for a further 48 hours. (B) Representative images of SKOV-3 cells stained using the senescence β-galactosidase staining kit following transfection for 72 hours (a-c) or 120 hours (d-f). (C) CCK-8 assay results. % cell viability for each condition was calculated as a percentage of untransfected SKOV-3 cells which were left untreated. The results demonstrate that knockdown of MAD2 renders SKOV-3 cells resistant to paclitaxel. (D) The percentage of β-galactosidase positive cells was calculated for each condition for (n = 3) technical and (n = 3) biological replicates. Following transfection a 3-fold increase in β-galactosidase expression was observed demonstrating that knockdown of MAD2 induces cellular senescence in SKOV-3 cells. (E) Trypan blue exclusion assay. (F) CCK-8 assay results for SKOV-3 cells treated with 200μM carboplatin for 48 hours following a 24 hour transfection. Results are expressed as mean +/-SD, n = 3. *p<0.05, **p<0.01, ***p<0.001 (Student’s t-test).
Powerlab, supplied by ADInstruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p-pak1(s199/204)/pak2(s192/197  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc p-pak1(s199/204)/pak2(s192/197
(A) Representative images of untransfected SKOV-3 cells and SKOV-3 cells transfected with siRNA targeting MAD2 (MAD2 siRNA) or a scrambled negative control siRNA (Scr siRNA) for 72 hours. After 24 hours, cells were either left untreated (-PTX) (a-c) or were treated with a 1μM dose of <t>paclitaxel</t> (+PTX) (d-f) for a further 48 hours. (B) Representative images of SKOV-3 cells stained using the senescence β-galactosidase staining kit following transfection for 72 hours (a-c) or 120 hours (d-f). (C) CCK-8 assay results. % cell viability for each condition was calculated as a percentage of untransfected SKOV-3 cells which were left untreated. The results demonstrate that knockdown of MAD2 renders SKOV-3 cells resistant to paclitaxel. (D) The percentage of β-galactosidase positive cells was calculated for each condition for (n = 3) technical and (n = 3) biological replicates. Following transfection a 3-fold increase in β-galactosidase expression was observed demonstrating that knockdown of MAD2 induces cellular senescence in SKOV-3 cells. (E) Trypan blue exclusion assay. (F) CCK-8 assay results for SKOV-3 cells treated with 200μM carboplatin for 48 hours following a 24 hour transfection. Results are expressed as mean +/-SD, n = 3. *p<0.05, **p<0.01, ***p<0.001 (Student’s t-test).
P Pak1(S199/204)/Pak2(S192/197, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-phospho-t402-srebp-1 antibody  (GenScript corporation)
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GenScript corporation anti-phospho-t402-srebp-1 antibody
(A) Representative images of untransfected SKOV-3 cells and SKOV-3 cells transfected with siRNA targeting MAD2 (MAD2 siRNA) or a scrambled negative control siRNA (Scr siRNA) for 72 hours. After 24 hours, cells were either left untreated (-PTX) (a-c) or were treated with a 1μM dose of <t>paclitaxel</t> (+PTX) (d-f) for a further 48 hours. (B) Representative images of SKOV-3 cells stained using the senescence β-galactosidase staining kit following transfection for 72 hours (a-c) or 120 hours (d-f). (C) CCK-8 assay results. % cell viability for each condition was calculated as a percentage of untransfected SKOV-3 cells which were left untreated. The results demonstrate that knockdown of MAD2 renders SKOV-3 cells resistant to paclitaxel. (D) The percentage of β-galactosidase positive cells was calculated for each condition for (n = 3) technical and (n = 3) biological replicates. Following transfection a 3-fold increase in β-galactosidase expression was observed demonstrating that knockdown of MAD2 induces cellular senescence in SKOV-3 cells. (E) Trypan blue exclusion assay. (F) CCK-8 assay results for SKOV-3 cells treated with 200μM carboplatin for 48 hours following a 24 hour transfection. Results are expressed as mean +/-SD, n = 3. *p<0.05, **p<0.01, ***p<0.001 (Student’s t-test).
Anti Phospho T402 Srebp 1 Antibody, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Representative images of untransfected SKOV-3 cells and SKOV-3 cells transfected with siRNA targeting MAD2 (MAD2 siRNA) or a scrambled negative control siRNA (Scr siRNA) for 72 hours. After 24 hours, cells were either left untreated (-PTX) (a-c) or were treated with a 1μM dose of paclitaxel (+PTX) (d-f) for a further 48 hours. (B) Representative images of SKOV-3 cells stained using the senescence β-galactosidase staining kit following transfection for 72 hours (a-c) or 120 hours (d-f). (C) CCK-8 assay results. % cell viability for each condition was calculated as a percentage of untransfected SKOV-3 cells which were left untreated. The results demonstrate that knockdown of MAD2 renders SKOV-3 cells resistant to paclitaxel. (D) The percentage of β-galactosidase positive cells was calculated for each condition for (n = 3) technical and (n = 3) biological replicates. Following transfection a 3-fold increase in β-galactosidase expression was observed demonstrating that knockdown of MAD2 induces cellular senescence in SKOV-3 cells. (E) Trypan blue exclusion assay. (F) CCK-8 assay results for SKOV-3 cells treated with 200μM carboplatin for 48 hours following a 24 hour transfection. Results are expressed as mean +/-SD, n = 3. *p<0.05, **p<0.01, ***p<0.001 (Student’s t-test).

Journal: PLoS ONE

Article Title: The role of the MAD2-TLR4-MyD88 axis in paclitaxel resistance in ovarian cancer

doi: 10.1371/journal.pone.0243715

Figure Lengend Snippet: (A) Representative images of untransfected SKOV-3 cells and SKOV-3 cells transfected with siRNA targeting MAD2 (MAD2 siRNA) or a scrambled negative control siRNA (Scr siRNA) for 72 hours. After 24 hours, cells were either left untreated (-PTX) (a-c) or were treated with a 1μM dose of paclitaxel (+PTX) (d-f) for a further 48 hours. (B) Representative images of SKOV-3 cells stained using the senescence β-galactosidase staining kit following transfection for 72 hours (a-c) or 120 hours (d-f). (C) CCK-8 assay results. % cell viability for each condition was calculated as a percentage of untransfected SKOV-3 cells which were left untreated. The results demonstrate that knockdown of MAD2 renders SKOV-3 cells resistant to paclitaxel. (D) The percentage of β-galactosidase positive cells was calculated for each condition for (n = 3) technical and (n = 3) biological replicates. Following transfection a 3-fold increase in β-galactosidase expression was observed demonstrating that knockdown of MAD2 induces cellular senescence in SKOV-3 cells. (E) Trypan blue exclusion assay. (F) CCK-8 assay results for SKOV-3 cells treated with 200μM carboplatin for 48 hours following a 24 hour transfection. Results are expressed as mean +/-SD, n = 3. *p<0.05, **p<0.01, ***p<0.001 (Student’s t-test).

Article Snippet: Carboplatin (C2538), Paclitaxel (T402) and DMSO (D2650) were purchased from Sigma Aldrich.

Techniques: Transfection, Negative Control, Staining, CCK-8 Assay, Expressing, Trypan Blue Exclusion Assay